The campus has two hospitals - one for adults and the dedicated children's facility
The Royal Hospital for Sick Children at Yorkhill has begun the process of moving to its new home in Glasgow.
The accident and emergency department closed at 08:00 and has transferred to the new Royal Hospital for Sick Children at the £842m southern campus.
As part of a phased move, inpatients, outpatient services and day surgery will transfer over the next four days.
The southern campus also contains the city's other new hospital - the South Glasgow University Hospital for adults.
Robert Calderwood, chief executive of NHS Greater Glasgow and Clyde, said: "The new adult acute hospital and new Royal Hospital for Sick Children have been a long time in the planning and construction, and I am delighted that they are now complete.
"The new Royal Hospital for Sick Children has been designed to the make any child's visit to the hospital as pleasant as possible with a cinema, science centre, interactive activity walls, indoor and outdoor play areas and a roof garden.
"We hope that the children who move to the new hospital this week are as pleased with it as we are."
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